北京译部落国际翻译有限公司

翻译样例中心 更多文章  样例中心

恶性肿瘤组织的氧化还原态、抗氧化能力的改变

2010-10-18 18:27

 

 

原文:氧化还原态的变化与肿瘤的发生、发展有着密切的关系,但目前相关研究对于肿瘤组织中此种变化尚无一致性结论。与正常组织相比较,癌组织的氧化还原态究竟发生了何种偏移?偏移程度多大?其抗氧化能力的变化如何?导致此种种改变的机制又如何呢?这几个问题的回答将对恶性肿瘤抗凋亡能力及其治疗提供新思路。方法:42例原发性消化道恶性肿瘤(食管癌13例、胃癌14例及结直肠癌15),以其相对应的癌旁正常粘膜组织为对照,组织匀浆测定其谷胱甘肽氧化还原对(GSHGSSG)含量及辅酶氧化还原对(NADPHNADP~+)的含量,并据此计算出GSH/GSSGNADPH/NADP~+比值以及GSH/GSSG氧化还原电位值、NADPH/NADP~+氧化还原电位值。同样的癌组织及相应对照组,集成于一张组织芯片上,以免疫组织化学技术检测凋亡相关因子Bcl-2BaxNF-κBcaspase-9的表达情况;并分析其表达与氧化还原态指标间的相关性。以探讨恶性肿瘤组织的氧化-还原态、抗氧化能力的改变与凋亡的关系及其可能机制。
译文:The alteration of the redox status in the tissue of malignant tumors has close relationship with carcinogenesis and cancer progression. The reports about the redox status of malignant tumors, however, remain controversial up -to-now. What direction of the redox status in cancer deviates to? And how much degree of the redox status changes are? And what is their antioxidative capability alteration? And what are the potential machanisms underlying these changes? Investigation of these questions may provide comprehensive knowledge about the anti-apptotic capability of malignant tumors and novel ideas about cancer therapy .Methods: The carcinoma tissues collected from 42 patients with primary cancer in digestive tract (13 cases of esophageal cancer 14 case of gastric cancer and 15 case of colorectal cancer), the corresponding paratumor mucosa tissues were taken as the control samples.After tissue homogenated, the content of oxidized and reduced glutathion(GSSG and GSH), oxidized and reduced coenzyme (NADP~+ and NADPH) were measured, the GSH/GSSG NADPH/NADP~+ ratios, and the GSH/GSSG NADPH/NADP~+ redox potentials were calculated according to Nernst formula. The same carcinoma tissues and the corresponding paratumor mucosa tissues were collected in a tissue chip. Then the expression of Bcl-2 Bax NF-κB caspase-9, factors involving in apoptosis, were determined by means of immunohistochemical staining, and the correlations between the expression and above indexes of redox status were analyzed.
 
 
 

2006-2012 北京译部落国际翻译有限公司 版权所有
Beijing E-buluo International Translation Co.,Ltd © All Rights Reserved http://www.transwins.com
公司地址:北京市朝阳区后现代城5号楼C座1101 电话:010-52894862 传真:010-58126862 手机:13681059052 E-mail:e-buluo@transguider.com
法律顾问:北京盈科律师事务所 刘卓 13910380132 DMOZ中文网站分类目录-免费收录各类优秀网站的中文网站目录. Coodir